在平菇生产中,广大菇农制作母种多是采用组织分离法。这种方法是将选好的种菇,在接种箱内用0.1％升汞表面消毒后,用解剖刀将菇体切开,在柄与盖交界处,切取一小块菌肉在试管培养基上培养。在生产实践中发现,采用此法分离即使严格按操作规则操作,成功率也只有20％左右。分析其失败原 In the production of mushrooms, mushroom production of the majority of the mother is the use of tissue separation method. This method is to choose the best kind of mushrooms in the inoculation box with 0.1% mercuric chloride surface disinfection, cut the mushroom body with a scalpel in the handle and the lid junction, cut a small piece of bacteria in the test tube medium On the training. Found in the practice of production, using this method even if strictly according to rules of operation, the success rate is only about 20%. Analysis of the original failure