本文建立了人血浆中汉防己甲素的高效液相色谱测定法。采用C18柱 ,以含 0 0 3%三乙胺的甲醇∶水 (80∶2 0 )为流动相 ,检测波长 2 82nm ,安定为内标 ,样品用乙醚提取。汉防己甲素线性范围 0 2 89～ 4.6 18μg·ml-1,平均加样回收率 95 8% ,日内及日间变异少于 5 %。方法简便、快速、可靠 ,可用于汉防己甲素的生物样本分析。 In this paper, a HPLC method for the determination of tetrandrine in human plasma was established. Using a C18 column, a methanol:water (80:20) containing 0.3% triethylamine was used as the mobile phase, the detection wavelength was 282 nm, and stability was used as the internal standard. The sample was extracted with ether. The linear range of tetrandrine was 0 2 89 to 4.6 18 μg·ml-1, with an average recovery of 95 8%. The intraday and intraday variation was less than 5%. The method is simple, rapid and reliable and can be used for the analysis of biological samples of tetrandrine.