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目的探讨磷脂酰肌醇蛋白聚糖-3(glypican-3,GPC3)在原发性肝癌中的表达及临床意义。方法采用免疫组化法检测45例手术切除的原发性肝癌及癌旁组织中GPC3的表达水平,比较原发性肝癌不同临床病理特征中GPC3的表达差异,分析GPC3表达与临床和病理因素的相关性。结果肝癌组织GPC3表达评分[(10.3±2.5)分]显著高于癌旁组织[(3.2±0.8)分],差异有统计学意义(P<0.05);不同性别、年龄组间GPC3蛋白表达比较,差异无统计学意义(P>0.05),而不同临床分期、肿瘤直径及有无转移患者比较,差异有统计学意义,GPC3在临床分期晚、肿瘤直径大、分化程度低及远处有转移的患者肝癌组织中表达强度增高(P<0.05)。logistic回归分析显示,PHC患者肝癌组织中GPC3表达与临床分期、肿瘤直径、分化程度及远处有转移等因素紧切相关(OR=0.623～0.960,P<0.05)。结论 GPC3蛋白在肝癌组织中呈高表达状态,对于其病情的诊断及预后评估具有重要价值。
Objective To investigate the expression of glypican-3 (GPC3) in primary hepatocellular carcinoma and its clinical significance. Methods Immunohistochemistry was used to detect the expression of GPC3 in 45 cases of primary hepatocellular carcinoma and adjacent normal tissues. The expression of GPC3 in different primary pathological features of primary hepatocellular carcinoma was analyzed. The expression of GPC3 was compared with the clinical and pathological factors Correlation. Results The expression of GPC3 in hepatocellular carcinoma was significantly higher than that in the adjacent tissues [(3.2 ± 0.8) [(10.3 ± 2.5) points] (P <0.05). The expression of GPC3 in different gender and age groups , There was no significant difference between the two groups (P> 0.05). However, there was significant difference between different clinical stages, tumor diameter and metastasis. GPC3 had late clinical stage, large tumor diameter, low differentiation and distant metastasis Of patients with hepatocellular carcinoma expression intensity increased (P <0.05). Logistic regression analysis showed that the expression of GPC3 in PHC patients was closely related to clinical stage, tumor diameter, differentiation degree and distant metastasis (OR = 0.623-0.960, P <0.05). Conclusion GPC3 protein is highly expressed in hepatocellular carcinoma, which is of great value for the diagnosis and prognosis evaluation of the disease.