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体外培养自体角质细胞皮片作为缺损皮肤永久性复盖对大面积烧伤病人(>40％TBSA)的治疗带来广阔的发展前景。以往10多年中,此种培养技术不断得到改进。本文报道与胰酶常规分离、培养法(Tr法)不同的热溶素(therm-olysin)改良法(Th-Tr法)。Tr法和Th-Tr法在制成细胞悬液、原代及传代培养方面并无差异,不同的只是,Tr法单用0.05％胰酶溶液在37℃消化削薄的成人皮肤标本;而Th-Tr法先用热溶素(500μg/ml)在37℃消化2小时,分离
In vitro culture of autologous keratinocyte skin flap as a permanent overlay of defect skin will bring broad prospects for the treatment of large area burn patients (> 40% TBSA). Over the past 10 years, such training techniques have been continuously improved. In this paper, the thermotolerance modified method (Th-Tr method), which is different from the conventional method of trypsin separation and culture method (Tr method), is reported. Tr method and Th-Tr method in the preparation of cell suspension, primary and subculture no difference, except that, Tr method with 0.05% trypsin digestion of thinned adult skin specimens at 37 ℃; Th The -Tr method was first digested with hot lysine (500 μg / ml) for 2 hours at 37 ° C and separated