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目的研究生后不同日龄小鼠喙端迁移流(RMS)的发育,神经干细胞增殖和凋亡的规律。方法利用Caspase-8免疫荧光标记法和5’-溴脱氧尿嘧啶核苷(BrdU)法,对小鼠RMS内的神经干细胞增殖和凋亡进行研究(n=92)。结果生后早期小鼠脑内,尤其是室管下区(SVZ)和RMS,存在大量的增殖细胞。随着小鼠年龄的增加,脑内干细胞逐渐减少,到成年,大脑皮质几乎见不到增殖的神经干细胞,但在SVZ和RMS仍可以看到许多增殖的神经干细胞。在RMS,神经干细胞增殖的同时伴随着细胞凋亡,干细胞的增殖与凋亡存在着正相关关系。结论 RMS的神经干细胞增殖与凋亡有重要的生理意义,通过细胞凋亡,RMS可以调节神经干细胞向嗅球迁移的数量,也可以调节干细胞向颗粒细胞分化。
OBJECTIVE: To study the development of beaked terminal flow (RMS), the proliferation and apoptosis of neural stem cells in mice of different ages. Methods Caspase-8 immunofluorescence staining and BrdU were used to study the proliferation and apoptosis of neural stem cells in mice RMS (n = 92). Results There were a large number of proliferating cells in the brain, especially in the subventricular zone (SVZ) and RMS in early postnatal mice. As mice age, brain stem cells gradually decreased to adulthood, the cerebral cortex almost no proliferation of neural stem cells, but in SVZ and RMS can still see a lot of proliferation of neural stem cells. In RMS, neural stem cell proliferation accompanied by apoptosis, there is a positive correlation between stem cell proliferation and apoptosis. Conclusion The proliferation and apoptosis of RMS neural stem cells have important physiological significance. By means of apoptosis, RMS can regulate the migration of neural stem cells to the olfactory bulb and regulate the differentiation of stem cells into granulosa cells.