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目的:研究OK-432与IL-2联用,抑制近交系小鼠C_(57)BL/6 Lewis肺癌的作用。方法:以G_(57)BL/6小鼠Lewis肺癌为模型,观察OK-432联合IL-2对肿瘤发生、生长、转移的影响,及体内对肿瘤细胞的作用。结果:二者联用对肿瘤体积和重量的抑制作用明显增强(P<0.01),增强抑瘤率(P<0.05),肺转移灶的数目明显减少。光镜下显示肿瘤灶中出现许多凋亡细胞;肿瘤组织电镜观察,瘤细胞核染色质浓集成块,在核膜内呈新月形或环形排列,核膜清楚,瘤细胞内质网扩张,线粒体肿胀。结论:在二者剂量减半的情况下,联合用药的抑瘤效应仍得到加强,克服了IL-2的毒副作用,其杀瘤过程主要为对瘤细胞的直接攻击,和促进体内多种反应细胞相互作用,诱导瘤细胞的凋亡。
OBJECTIVE: To study the effect of OK-432 in combination with IL-2 to inhibit C57BL / 6 Lewis lung carcinoma in mice of inbred mice. Methods: The Lewis lung carcinoma cell line G_ (57) BL / 6 was used as a model to observe the effect of OK-432 combined with IL-2 on tumorigenesis, growth and metastasis and the effect on tumor cells in vivo. Results: The combination of the two drugs significantly inhibited tumor volume and weight (P <0.01), enhanced tumor inhibition rate (P <0.05), and significantly reduced the number of lung metastases. Under the light microscope, many apoptotic cells appeared in the tumor focus. The electron microscopy showed that the chromatin of the tumor cells concentrated into clumps, crescent or annular arrangement in the nuclear membrane, clear nuclear membrane, expansion of the endoplasmic reticulum of the tumor cells, mitochondria swelling. Conclusions: The antitumor effect of combination therapy is still enhanced when the doses of the two are reduced by half, which overcomes the toxic and side effects of IL-2. The killing process is mainly direct attack on tumor cells and promotion of multiple reactions in vivo Cell interaction, induce apoptosis of tumor cells.