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在保存疟原虫中,许多科学家都使用液氮(-196℃)。在冷冻-解冻过程中,对疟原虫活力有影响的因素是:冷冻与解冻的速度,冷冻保护剂的类型和复苏的方法。为了评价低温保存恶性疟原虫培养物的效果,本文进行了两种冷冻方法和三种复苏方法的比较研究。所用的恶性疟原虫株是D/BN/IMP82I,自1例在肯尼亚感染的病例分离。原虫培养物用500g离心7分钟,弃上清,与9倍量的5%山梨醇混合,在37℃孵育20分钟,再次离心,再用RPMI-Hepes-NaHCO_3培养基
Many scientists use liquid nitrogen (-196 ° C) to conserve the parasite. Factors that affect the viability of Plasmodium during freezing-thawing are the speed of freezing and thawing, the type of cryoprotectant and the method of recovery. To evaluate the effect of cryopreserved P. falciparum cultures, a comparative study of two cryogenic and three resuscitative methods was performed. The P. falciparum strain used was D / BN / IMP82I, isolated from one case of infection in Kenya. Protozoal cultures were centrifuged at 500 g for 7 minutes, the supernatant was discarded, mixed with 9 times the amount of 5% sorbitol, incubated at 37 ° C for 20 minutes, centrifuged again, and re-suspended in RPMI-Hepes-NaHCO 3 medium