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目的 在Light-CyclePCR仪上对多药耐药基因 (MDR1)定量的技术方法和临床应用进行探讨。方法 应用荧光定量逆转录 -多聚酶链反应 (RT -PCR)检测了 30例急性白血病患者和 8例正常人外周血MDR1基因的表达。结果 该实验方法标准曲线线性良好 (r=1.0 ) ,耐药组MDR1基因拷贝数与非耐药组有显著性差异 (P <0 .0 1) ,正常人亦有低水平MDR1基因的表达。结论 荧光逆转录 -多聚酶链反应 (RT -PCR)是一种可靠的定量方法 ,能快速、特异地检测临床肿瘤标本中MDR1,为临床医生用药、预防MDR发生、考核逆转MDR效果等方面提供了有价值的量化指标。
Objective To investigate the technique and clinical application of Quantitative Multidrug Resistance (MDR1) in Light-Cycle PCR. Methods The expression of MDR1 gene in peripheral blood of 30 patients with acute leukemia and 8 normal controls was detected by RT-PCR. Results The standard curve of the experimental method was linear (r = 1.0). The MDR1 gene copy number in drug-resistant group was significantly different from that in non-drug-resistant group (P <0.01), and the expression of MDR1 gene was also low in normal subjects. Conclusion RT-PCR is a reliable quantitative method for rapid and specific detection of MDR1 in clinical tumor samples, providing clinicians with medication, preventing the occurrence of MDR, and reversing the effect of MDR Valuable quantitative indicators.