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目的 探讨胶质瘤细胞ING1基因表达水平及其与肿瘤细胞增殖活性和凋亡程度的关系。方法 采用原位杂交及免疫组织化学染色方法 ,分别检测 71例不同级别的人胶质瘤组织ING1mRNA及ING 1、PCNA蛋白 ,并采用TUNEL法检测原位细胞凋亡的情况。结果 ING 1mRNA的阳性表达率为 94.6% (3 5 / 3 7) ,ING 1蛋白的阳性表达率为 90 .1% (64 / 71) ,两者的表达水平呈正相关 (γ =0 .714 ,P <0 .0 1) ,均随肿瘤恶性程度的增高而降低 ,具有显著性差异 (P <0 .0 5 )。ING 1表达水平降低时伴随着凋亡程度的降低及增殖活性的增强。结论 胶质瘤细胞中ING1基因的表达水平与肿瘤恶性程度密切相关 ,并随肿瘤恶性程度的增加而相应降低。其表达异常的改变主要是转录水平调控异常的结果 ,并可能通过促进细胞增殖和抑制细胞凋亡 ,在胶质瘤的发生、发展中发挥重要作用。
Objective To investigate the expression of ING1 gene in glioma cells and its relationship with the proliferation of tumor cells and the degree of apoptosis. Methods In situ hybridization and immunohistochemical staining were used to detect ING1 mRNA, ING1 and PCNA protein in 71 cases of different grades of human glioma respectively. TUNEL method was used to detect the apoptosis in situ. Results The positive rate of ING 1 mRNA was 94.6% (35/37) and the positive rate of ING 1 protein was 90.1% (64/71). There was a positive correlation between the expression of ING 1 mRNA (γ = 0.714, P <0.01), with the malignant degree of tumor decreased, with significant difference (P <0.05). The decrease of ING-1 expression accompanied with the decrease of apoptosis and the increase of proliferative activity. Conclusions The expression level of ING1 in glioma cells is closely related to the degree of malignancy of tumor, and it is correspondingly decreased with the increase of malignant degree of tumor. The abnormal expression changes are mainly the result of aberrant transcriptional regulation and may play an important role in the development of gliomas by promoting cell proliferation and inhibiting apoptosis.