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目的:探讨宫颈上皮内瘤变证候特征和宫颈免疫因子TNF-α的内在联系,为临床治疗宫颈上皮内瘤变(Cervical intraepithelial neoplasia,CIN)提供依据.方法:本次研究采取回顾性分析的方法,选取2015年2月到2017年2月在本院行宫颈上皮内瘤变治疗的患者86例,应用免疫组织化学SP法检测患者宫颈组织标本中TNF-α的阳性表达,通过图象分析软件对TNF-α的阳性表达进行半定量分析.结果:86例CIN患者中,其中CIN Ⅰ有34例,CIN Ⅱ有28例,CIN Ⅲ有24例,CIN Ⅰ患者多属于脾虚湿盛型,CIN Ⅱ患者多属于湿热内蕴型,CIN Ⅲ患者也多属于湿热内蕴型;TNF-α在三种级别的患者的组织中表达的IOD值分别为4.097±1.501、6.286±1.428、7.601士1.470,差异具有统计学意义(P＜0.05);不同级别的CIN病变中TNF-α的表达强度与宫颈病变程度呈正相关关系;TNF-αIOD值在各种类型的CIN中有明显的趋势,其中脾虚湿盛型值最高,肝郁气滞型值最低,差异具有统计学意义(P＜0.05).结论:TNF-αdOD值与宫颈病变程度呈正相关关系,宫颈免疫因子TNF-α的表达程度对中医分型有一定的参考价值,可以为阻断宫颈上皮内瘤变提供依据.“,”Objective:To study the intrinsic relationship between cervical intraepithelial neoplasia syndrome and cervical immune factor TNF-o,and to provide the basis for clinical treatment of cervical intraepithelial neoplasia (cervical intraepithelial neoplasia,CIN).Methods:A retrospective analysis was conducted in this study.86patients with cervical intraepithelial neoplasia from February 2015to February 2017were selected.Immunohistochemical SP method was used to detect the expression of TNF-opositive expression,semi-quantitative analysis of TNF-α positive expression by image analysis software.Results:Among the 86cases of CIN,34cases were CIN Ⅰ,28cases were CIN Ⅱ,24cases were CIN Ⅲ,CIN Ⅰ patients were mostly spleen dampness type,CIN Ⅱ patients were mostly damp-heat type,CIN Ⅲ patients.The IOD valuesof TNF-α were 4.097±1.501,6.286±1.428and 7.601 ± 1.470,respectively,and the difference was statistically significant (P＜0.05).There was a positive correlation between the expression of TNF-α and the degree of cervical lesions in the CIN lesions.The TNF-αIOD values showed a significant trend in various types of CIN,with the highest spleen dampness value and the lowest,the difference was statistically significant (P＜0.05).Conclusion:The level of TNF-αIOD is positively correlated with the degree of cervical lesions.The expression of TNF-o is of great reference value to the classification of cervical intraepithelial neoplasia.