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目的:研究浸润于肿瘤组织中的CD4+T淋巴细胞与自身B细胞非霍奇金淋巴瘤(B-NHL)肿瘤细胞间的相互作用。方法:采用磁珠抗体分离法将1例B-NHL患者的脾边缘区恶性B淋巴细胞与浸润在肿瘤组织中的CD4+T细胞分离出来,共培养并观察恶性B淋巴细胞的生物学变化。结果:恶性的B细胞发生了形态学变化,逐渐向浆细胞转化;同时,逐渐丧失其细胞膜表面特异性标记CD19等B细胞特征性的标记,取而代之的是CD138等成熟浆细胞特有的标记分子。通过抗体分离得到CD138+细胞后,经重组免疫球蛋白(Ig)重链的PCR进一步证实这些细胞来源于恶性B细胞。结论:低分化恶性B细胞的正常分化过程并非完全阻断,适当的外界微环境能启动受阻的分化过程。肿瘤组织来源的CD4+T细胞具有促使自体肿瘤B细胞向浆细胞分化的能力。
OBJECTIVE: To study the interaction between CD4 + T lymphocytes infiltrating tumor tissue and B-cell non-Hodgkin’s lymphoma (B-NHL) tumor cells. Methods: The malignant B lymphocytes in one patient with B-NHL were separated from CD4 + T cells infiltrated in the tumor tissue by magnetic bead antibody isolation method. The biological changes of malignant B lymphocytes were observed and co-cultured. Results: The morphological changes of malignant B cells gradually transformed into plasma cells. At the same time, the malignant B cells gradually lost the characteristic markers of B cells such as CD19 on the surface of cell membrane, and replaced with markers specific to mature plasma cells such as CD138. After isolation of CD138 + cells by antibodies, PCR with recombinant immunoglobulin (Ig) heavy chains further confirmed that these cells were derived from malignant B cells. Conclusion: The normal differentiation of poorly differentiated malignant B cells is not completely blocked, and appropriate external microenvironment can initiate the hindered differentiation process. Tumor tissue-derived CD4 + T cells have the ability to induce autologous tumor B cells to differentiate into plasma cells.