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通过菌株生态定殖以及抗病物质分析,初步探究解淀粉芽胞杆菌(Bacillus amyloliquefaciens)YH-22抗青枯病(茄科雷尔氏菌所致)的机制。采用利福平抗性诱导,获得抗400μg/mL的抗性菌株以利于定殖研究。盆栽试验结果表明,YH-22先在烟草根际土壤定殖,然后依次进入根部、茎部。对YH-22菌株发酵上清液进行理化性质分析,发现抗茄科雷尔氏菌活性物质不溶于氯仿和乙酸乙酯,能溶于甲醇,具有一定的耐热性,能耐受胃蛋白酶和紫外线,具有排油和使液滴坍塌的特性。综上所述,生防菌YH-22在烟草根际土壤、根、茎均具有一定的定殖能力,其抗菌物质可能为蛋白质和脂肽的混合物。
The mechanism of Bacillus amyloliquefaciens YH-22 resistance to bacterial wilt (caused by Ralstonia solanacearum) was explored through ecological colonization and analysis of disease-resistant substances. Using rifampicin resistance induction, resistant strains resistant to 400 μg / mL were obtained to facilitate colonization research. The results of pot experiment showed that YH-22 was first colonized in tobacco rhizosphere so as to enter roots and stems. The physical and chemical properties of YH-22 strain fermentation supernatant were analyzed and found that active substances resistant to R. solanacearum insoluble in chloroform and ethyl acetate, soluble in methanol, has a certain heat resistance, can withstand pepsin and UV, with oil discharge and droplet collapse characteristics. In summary, biocontrol strain YH-22 has certain colonization ability in rhizosphere soil, root and stem of tobacco, and its antibacterial substance may be a mixture of protein and lipopeptide.