迄今已有不少文献证明,巨噬细胞和自然杀伤细胞是在体内抗肿瘤生长中起免疫监视作用的两种效应细胞。此外观察发现,肿瘤坏死因子-α(TNF-α)在巨噬细胞介导的抗瘤反应中也起强效应因子的作用,这样便为进一步研究体内肿瘤形成和体外肿瘤细胞生长的调节提供了理论依据。另有报道证明生长因子既有协同作用,又有抵抗作用,很多转化细胞分泌的因子能通过自身机制刺激生长。为了验证纯化的生 Up to now, there have been many documents that macrophages and natural killer cells are two kinds of effector cells that play a role of immune surveillance in anti-tumor growth in vivo. In addition, it was observed that tumor necrosis factor-alpha (TNF-alpha) also plays a role as a strong effector factor in macrophage-mediated antitumor responses, thus providing a further study of in vivo tumorigenesis and regulation of tumor cell growth in vitro. Theoretical basis. It has also been reported that growth factors have both synergistic and resistance effects and that many factors secreted by transformed cells can stimulate growth through their own mechanisms. In order to verify the purification of raw