论文部分内容阅读
本研究探讨骨髓源间充质干细胞对单倍体相合异基因造血干细胞移植后患者造血微环境的影响。选择间充质干细胞联合造血干细胞移植的15名患者为研究组,单纯造血干细胞移植的20名患者为对照组,抽取35名患者移植后不同时期骨髓标本47份,进行MSC培养,观察MSC能达到原代融合的例数及其融合时间;从移植前开始抽取患者外周血以检测中性粒细胞和血小板的水平;每隔4天留取患者外周血浆标本,用ELISA检测SDF-1α、TPO及IL-11的浓度,观察两组之间浓度动态变化的差异。结果表明,研究组MSC体外原代融合率(60%)明显高于对照组(27.3%)(p<0.01),且两组原代融合时间分别为27.13±4.41天与36.09±11.84天(p<0.05)。回输组SDF-1α浓度在移植第8天达最高峰(2975.19±681.56pg/ml),较移植前(2403.70±522.39pg/ml)升高(p<0.05),对照组SDF-1α浓度在移植后第16天达最大值(2280.60±701.25pg/ml),较移植前(2701.46±483.21pg/ml)减少(p<0.05)。从移植后第16天至第28天,研究组TPO和IL-11的浓度均高于对照组(p<0.05)。结论:联合间充质干细胞移植能明显改善单倍体相合异基因造血干细胞移植患者骨髓微环境的损伤状态。
This study was to investigate the effect of bone marrow-derived mesenchymal stem cells on hematopoietic microenvironment after haploidentical allogeneic hematopoietic stem cell transplantation. Fifteen patients with mesenchymal stem cells combined with hematopoietic stem cell transplantation were selected as study group and 20 patients with simple hematopoietic stem cell transplantation as control group. Forty-seven bone marrow samples from 35 patients at different stages after transplantation were collected and cultured in MSC. The number of primary fusion and the fusion time were taken. Peripheral blood samples were taken from the patients before transplantation to detect neutrophils and platelets. Peripheral blood plasma samples were collected every 4 days. The levels of SDF-1α, TPO and IL-11 concentrations were observed between the two groups the dynamic changes in concentration differences. The results showed that the in vitro primary fusion rate (60%) of study group was significantly higher than that of control group (27.3%) (p <0.01), and the primary fusion time was 27.13 ± 4.41 days and 36.09 ± 11.84 days <0.05). The concentration of SDF-1α in the transfusion group reached the peak (2975.19 ± 681.56pg / ml) on the 8th day of transplantation, which was higher than that before transplantation (2403.70 ± 522.39pg / ml) (p <0.05) The maximum value (2280.60 ± 701.25pg / ml) on the 16th day after transplantation was decreased compared with that before transplantation (2701.46 ± 483.21pg / ml) (p <0.05). From the 16th day to the 28th day after transplantation, the concentrations of TPO and IL-11 in the study group were higher than those in the control group (p <0.05). Conclusion: MSCs transplantation can significantly improve the bone marrow microenvironment injury in haploidentical allogeneic hematopoietic stem cell transplantation patients.