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目的比较辐照致弱日本血吸虫尾蚴免疫小鼠与正常感染小鼠早期免疫活化程度及其动态变化差异。方法采用流式细胞术(FCM)和免疫组织化学(IHC)方法比较辐照致弱尾蚴免疫小鼠与正常感染小鼠早期脾组织和/或肺组织中树突状细胞(DC)表面分子CD11c、T细胞表面分子CD25表达差异及脾细胞和外周血中CD3+CD25+/CD3+T细胞比例,分析T细胞免疫活化程度及其动态变化。结果在感染后7d,脾细胞中的CD3+CD25+/CD3+T细胞比例致弱尾蚴免疫组为(19.52±3.65)%,明显低于正常感染组的(22.12±3.24)%;而在第14、21天,致弱尾蚴免疫组这一比例分别为(28.73±3.94)%和(26.43±0.40)%,均高于正常感染组的(13.68±3.64)%和(14.42±2.24)%。在攻击感染后7、14、21d,致弱尾蚴免疫组与正常感染组小鼠在肺组织中的CD11c+DC表达率分别为(1.05±0.16)%和(0.96±0.15)%、(1.34±0.15)%和(1.09±0.17)%、(1.49±0.14)%和(0.97±0.16)%,脾组织中CD11c+DC表达率分别为(2.05±0.26)%和(1.95±0.18)%、(2.24±0.25)%和(2.17±0.25)%、(2.18±0.26)%和(2.06±0.18)%。在攻击感染后7、14、21d,致弱尾蚴免疫组与正常感染组小鼠在肺组织中CD25+T细胞表达率分别为(1.24±0.13)%和(1.17±0.16)%、(1.48±0.11)%和(1.25±0.13)%、(1.55±0.14)%和(0.97±0.12)%,脾组织中CD25+T细胞表达率分别为(3.25±0.22)%和(2.93±0.20)%、(4.57±0.23)%和(3.69±0.24)%、(4.28±0.24)%和(3.86±0.26)%,紫外线辐照致弱尾蚴免疫小鼠较正常感染小鼠在攻击感染后第7、14、21天能在肺组织募集更多的CD11c+DC并激活更多的CD25+T细胞。结论致弱日本血吸虫尾蚴免疫小鼠在攻击感染后14、21d,小鼠体内T细胞激活程度和肺部DC活化程度均高于正常感染组,提示致弱尾蚴在肺部可募集更多抗原递呈细胞并使之活化。
OBJECTIVE: To compare the difference of early immunological activation and the dynamic changes between mice immunized with cercariae of Schistosoma japonicum and normal mice. Methods Flow cytometry (FCM) and immunohistochemistry (IHC) were used to compare the expression of CD11c on the surface of dendritic cells (DCs) in mice immunized with irradiated cercariae with normal mice. , The difference of T cell surface molecule CD25 expression and the proportion of CD3 + CD25 + / CD3 + T cells in spleen cells and peripheral blood were analyzed. The degree of T cell immune activation and its dynamic changes were analyzed. Results At 7 days after infection, the percentage of CD3 + CD25 + / CD3 + T cells in splenocytes was (19.52 ± 3.65)% in immunocompromised group, which was significantly lower than that in normal infection group (22.12 ± 3.24)%, while in the 14th (28.73 ± 3.94)% and (26.43 ± 0.40)% respectively in the immunodeficiency group and 21.6 days in the control group (13.68 ± 3.64)% and (14.42 ± 2.24)%, respectively. The expression rates of CD11c + DC in the lungs of mice immunized with weak cercariae and normal mice at 7, 14 and 21 days after challenge were (1.05 ± 0.16)% and (0.96 ± 0.15)%, (1.34 ± (2.05 ± 0.26)% and (1.95 ± 0.18)%, (1.09 ± 0.17)%, (1.49 ± 0.14)% and (0.97 ± 0.16)%, respectively 2.24 ± 0.25% and 2.17 ± 0.25%, 2.18 ± 0.26% and 2.06 ± 0.18% respectively. The positive rates of CD25 + T cells in the lungs of mice immunized with weak cercariae and normal mice at 7, 14 and 21 days after challenge were (1.24 ± 0.13)% and (1.17 ± 0.16)%, (1.48 ± (3.25 ± 0.22)% and (2.93 ± 0.20)% respectively in the spleen tissues were significantly higher than those in the control group (0.11%) and (1.25 ± 0.13)%, (1.55 ± 0.14)% and (0.97 ± 0.12) (4.57 ± 0.23)% and (3.69 ± 0.24)%, (4.28 ± 0.24)% and (3.86 ± 0.26)%, respectively. Compared with the mice infected by UV irradiation, , 21 days to recruit more CD11c + DC in lung tissue and activate more CD25 + T cells. Conclusion The activation of T cells and activation of lung in mice immunized with cercariae of Schistosoma japonicum at 14 and 21 days after challenge were higher than those in normal mice, suggesting that the cercariae could attract more antigen in the lung Presented and activated.