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利用本室生产的二株抗人血小板单克隆抗体SZ-21和SZ-22,研制成功能够检测抗血小板膜糖蛋白(GP)Ⅱb和Ⅲa自身抗体的酶联免疫测定药盒。利用SZ-21或SZ-22(分别作用于GPⅢa和GPⅡb)包被的微孔结合正常血小板提取液中的GPⅡb/Ⅲa,洗涤后加入病人或正常对照的血浆,然后以辣根过氧化物酶联结的抗人IgG检测抗GPⅡb/Ⅲa的自身抗体。应用该药盒检测69例原发性血小板减少性紫癜(ITP)患者血浆中抗GP直Ⅱb/Ⅲa自身抗体,其中22例为阳性,阳性率31.8%。急性与慢性ITP的抗GPⅡb/Ⅲa抗体阳性率无差别,但儿童ITP组抗GPⅡb/Ⅲa抗体阳性率(20%)低于成人ITP组的阳性率(41.0%)。抗GPⅡb/Ⅲa抗体的阳性结果与PAIgG含量无相关,与血小板数也无相关。
Using the two anti-human platelet monoclonal antibodies SZ-21 and SZ-22 produced in our laboratory, an ELISA kit capable of detecting anti-platelet glycoprotein (GP) IIb and IIIa autoantibodies was successfully developed. The microplates coated with SZ-21 or SZ-22 (acting on GPIIIa and GPIIb, respectively) were bound to GPIIb / IIIa in normal platelet extract, washed and added to the plasma of patients or normal controls, followed by horseradish peroxidase Bound anti-human IgG detects anti-GPIIb / IIIa autoantibodies. The kit was used to detect the plasma anti-GP Ⅱb / Ⅲa autoantibodies in 69 patients with idiopathic thrombocytopenic purpura (ITP), of which 22 were positive and the positive rate was 31.8%. The positive rates of anti-GPⅡb / Ⅲa antibody in acute and chronic ITP were not different. However, the positive rate of anti-GPⅡb / Ⅲa antibody in children with ITP was lower than that in adult ITP (41.0%). The positive result of anti-GPⅡb / Ⅲa antibody had no correlation with the content of PAIgG and no correlation with the number of platelets.