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目的研究苯并(a)芘诱导人胚肺(ccc HPF1)细胞DNA损伤修复中基因JWA和热休克蛋白(hsp70)的表达规律,探讨JWA基因参与细胞DNA损伤修复的作用和可能机制。方法建立ccc HPF1细胞DNA损伤模型,在S9活化状态下分别以10～100μmol/L苯并(a)芘处理细胞3h收获细胞或再恢复24h,用碱性单细胞凝胶电泳鉴定DNA损伤和修复情况,免疫印迹方法检测JWA和hsp70的表达水平。结果在DNA损伤模型中,苯并(a)芘活跃地调节JWA的表达,50μmol/L和100μmol/L处理组JWA和hsp70明显上调,在恢复期内10～100μmol/L处理组两者都处于较高的表达水平并且JWA和hsp70的表达规律几乎完全一致。结论JWA是一个有效的环境应答基因,活跃地参与细胞应答与DNA切除修复有关的信号通路。
Objective To investigate the expression of genes JWA and heat shock protein (hsp70) induced by benzo (a) pyrene in DNA damage repair of human embryo lung (ccc HPF1) cells and to explore the role and possible mechanism of JWA gene involved in cell DNA damage repair. Methods The DNA damage model of ccc HPF1 cells was established. Cells were treated with 10-100 μmol / L benzo (a) pyrene for 3 hours under S9 activation condition, and then harvested or resumed for 24 hours. DNA damage and repair were identified by alkaline single cell gel electrophoresis Western blotting was used to detect the expression of JWA and hsp70. Results In the DNA damage model, benzo (a) pyrene actively regulated JWA expression. JWA and hsp70 were significantly up-regulated in 50μmol / L and 100μmol / L treatment groups, both in 10-100μmol / L treatment group Higher expression levels and almost identical expression pattern of JWA and hsp70. Conclusion JWA is a potent environmental response gene that is actively involved in the signaling pathways involved in cellular responses to DNA excision and repair.