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目的:建立乙肝解毒口服液中柯里拉京和没食子酸含量测定方法。方法:采用高效液相色谱法,Diamonsil C18(4.6mm×150mm,5μm)色谱柱,乙腈-0.1%磷酸(1288)为流动相,流速1.0mL·min-1,柱温30℃,检测波长274nm。结果:柯里拉京线性范围0.274～1.918μg(r=0.9999),平均回收率96.55%(n=6);没食子酸线性范围0.196～1.372μg(r=0.9999),平均回收率97.91%(n=6)。结论:该法快速、简便、准确、专属性强,可用于乙肝解毒口服液中柯里拉京和没食子酸的含量测定。
Objective: To establish a method for the determination of corilagin and gallic acid in the oral solution of Methods: High performance liquid chromatography (HPLC) was performed on a Diamonsil C18 column (4.6 mm × 150 mm, 5 μm) with acetonitrile-0.1% phosphoric acid (1288) as mobile phase at a flow rate of 1.0 mL · min- Wavelength 274nm. The linear range of corilagin was 0.274-1.918μg (r = 0.9999), the average recovery was 96.55% (n = 6). The linear range of gallic acid was 0.196-1.372 g (r = 0.9999), the average recovery was 97.91% 6). Conclusion: The method is rapid, simple, accurate and specific. It can be used for the determination of corilagin and gallic acid in the oral solution of.