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目的探讨核因子κB1(NF-κB1)基因启动子-94ins/del ATTG基因多态性与动脉粥样硬化性脑梗死(ACI)的关系。方法采用PCR限制性片段长度多态性分析(PCR-RFLP)法检测191例ACI患者(其中中国东北地区ACI患者101例,湖北省ACI患者90例)和171名健康对照(其中东北地区90名,湖北省81名)的NF-κB 1基因启动子-94ins/del ATTG基因型,分析该基因多态性与ACI的关系。结果 ACI组患者的NF-κB1-94ins/del ATTG 2种基因型频率(WW和DD型)和2种等位基因频率(W和D型)与健康对照者间比较有统计学差异(P<0.01);东北地区健康对照组与东北ACI组DD基因型频率和等位基因频率(W和D型)存在统计学差异(P<0.05),湖北地区健康对照组与ACI组WW、DD基因型频率和等位基因频率(W和D型)存在统计学差异(P<0.05),两地区健康对照组之间比较以及ACI组间比较均无统计学差异(P>0.05)。结论 NF-κB1基因启动子-94ins/del ATTG基因多态性与ACI发病有一定相关性,但其相关性不存在地区差异。
Objective To investigate the relationship between the-94ins / del ATTG gene polymorphism of NF-κB1 gene promoter and atherosclerosis infarction (ACI). Methods PCR-RFLP was used to detect 191 ACI patients (101 ACI patients in Northeast China, 90 ACI patients in Hubei Province) and 171 healthy controls (including 90 in Northeast China , 81 in Hubei province) of NF-κB 1 gene promoter -94ins / del ATTG genotype, analysis of the relationship between gene polymorphism and ACI. Results The frequencies of NF-κB1-94ins / del ATTG genotypes (WW and DD genotypes) and two allelic frequencies (W and D genotypes) in ACI group were significantly different from those in healthy controls (P < 0.01). DD genotype frequency and allele frequency (W and D type) in northeastern region healthy control group and northeast ACI group were significantly different (P <0.05), while WW and DD genotypes in healthy control group and ACI group in Hubei Province The frequency and allele frequency (W and D type) had statistical difference (P <0.05). There was no significant difference between the two control groups (P> 0.05). Conclusion The polymorphism of NF-κB1 gene promoter-94ins / del ATTG has some correlation with the onset of ACI, but there is no regional difference in its correlation.