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以木薯腋芽为外植体,通过添加不同含量的2,4-D和picloram摸索诱导体细胞胚及FEC的最佳激素条件;利用优化的再生体系,以木薯脆性胚性愈伤组织(FEC)为受体,对农杆菌侵染FEC的侵染浓度、侵染时间以及侵染后潮霉素抗性筛选浓度进行了研究。结果表明,12 mg·L~(-1)的picloram能较快诱导出体细胞胚及FEC;农杆菌菌液浓度OD600=0.4并侵染30 min为FEC转化效率及植株再生率的最佳条件;共培养后,通过逐渐增加潮霉素筛选浓度(0,5,8,15 mg·L~(-1))能更有效地提高植株的再生率。
Using cassava axillary bud as explant, the optimum hormone conditions for somatic embryogenesis and FEC induction were explored by adding different contents of 2,4-D and picloram. Using optimized regenerated system, the embryogenic callus (FEC) As the recipient, the infection concentration of Agrobacterium tumefaciens FEC, the infection time and the concentration of hygromycin resistance after inoculation were studied. The results showed that 12 mg · L -1 picloram could rapidly induce somatic embryogenesis and FEC. The optimal conditions of FEC transformation efficiency and plant regeneration rate were obtained when the concentration of Agrobacterium was OD600 = 0.4 and the infection time was 30 min After the co-culture, the regeneration rate of plants can be more effectively increased by gradually increasing the screening concentration of hygromycin (0, 5, 8 and 15 mg · L -1).