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采用阻断大鼠双侧椎动脉和颈总动脉30分钟再灌流4小时的方法,把实验动物随机分为给药组(注射山茛菪碱)与实验组(注射等量生理盐水)及假手术对照组。观察各组动物实验前、后的脑电图变化; 实验后脑组织的水、纳、钾含量的变化及光镜和电镜下的形态学改变;并统计活存数。结果:实验组的上述各项指标与假手术对照组之间呈现高度显著差异性(P<0.01),表明实验组已构成明显的脑缺血再灌流损伤。给药组的上述各项指标与实验组之间的差异为高度显著性(P<0.01),而与假手术对照组比较却未呈现显著性差异(P>0.05)。提示山茛菪碱在大鼠急性脑缺血再灌流的损伤过程中发挥了保护效应。
After blocking the bilateral vertebral artery and common carotid artery of the rat for 30 minutes and reperfusion for 4 hours, the experimental animals were randomly divided into the administration group (injection of anisodamine) and the experimental group (injection of the same amount of saline) and the sham Surgical control group. Observe the electroencephalogram changes before and after the experiment in each group of animals; change the water, sodium and potassium contents of the brain tissue after the experiment, and the morphological changes under the light microscope and the electron microscope; and count the survival number. RESULTS: There was a highly significant difference between the above-mentioned indicators of the experimental group and the sham-operated control group (P<0.01), indicating that the experimental group has constituted significant cerebral ischemia-reperfusion injury. The difference between the above-mentioned indicators and the experimental group in the administration group was highly significant (P<0.01), but there was no significant difference compared with the sham control group (P>0.05). It is suggested that anisodamine exerts a protective effect during acute cerebral ischemia and reperfusion in rats.