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目的 :运用大鼠CIA模型 ,观察造模后早期给药通痹灵治疗对大鼠软骨病理结构改变的影响作用 ,并通过与免疫抑制剂MTX、雷公藤多甙片的对比研究探讨中医药在治疗类风湿性关节炎软骨破坏中的可能机制。方法 :取健康清洁级 10 0g± 2 0g雄性Wistar大鼠 4 8只 ,随机纳入正常组 ,模型组 ,甲胺碟呤低、高组 ,通痹灵低、高组 ,雷公藤低、高组 ,于CIA造模后 10d分别给药 36d。采集各组大鼠膝关节切HE片对软骨缺损深度、软骨缺损范围、炎细胞浸润、纤维组织增生、软骨下骨组织反应性增生 5项指标严格分四级进行评价。结果 :试验结果发现 ,通痹灵高剂量在减少软骨的破坏以及抑制炎症细胞浸润、纤维组织增生上较为突出 ,其炎细胞浸润、纤维组织增生、软骨下骨组织反应性增生三项指标最接近正常组 ,不但与造模组比较有统计学差异 (P <0 0 5 ) ,还优于MTX等组(P <0 0 5 ) ,MTX高组也表现出一定的抑制软骨的破坏及纤维组织增生作用 ,并与MTX低组一样呈现出活跃的软骨下骨组织反应性增生。结论 :通痹灵在类风湿关节炎模型的软骨及骨破坏中能有效地抑制病变周围组织炎细胞的浸润和纤维组织增生 ,并降低和减少了软骨破坏的程度使软骨结构能保持接近正常组织的形态 ,并优于MTX及雷公藤多甙等药 ,具有良好的治疗和改善作用
OBJECTIVE: To observe the effect of Tongbiling administration on the pathological changes of rat cartilage after rat model was established by using a rat CIA model, and to investigate the effect of traditional Chinese medicine in comparison with immunosuppressive MTX and triptolide. Possible mechanisms in treating cartilage destruction in rheumatoid arthritis. METHODS: Forty-eight male Wistar rats with healthy and clean grades of 10 0g ± 2 0g were randomly included in the normal group, the model group, the low- and high-rate groups of methotrexate, the low and high groups of Tongfuling, and the low and high groups of Tripterygium wilfordii. , 36 days after the CIA model was administered for 36 days. The scores of cartilage defect, cartilage defect, inflammatory cell infiltration, fibrous tissue hyperplasia, and reactive hyperplasia of subchondral bone in five groups were all evaluated in four groups. RESULTS: The results showed that high doses of Tongqiaoling were more prominent in reducing cartilage destruction, inhibiting inflammatory cell infiltration, and fibrous tissue hyperplasia. The three indicators of inflammatory cell infiltration, fibrous tissue hyperplasia, and subchondral bone tissue reactive hyperplasia were the closest. The normal group was not only statistically different from the model group (P < 0.05), but also superior to the MTX group (P <0.05). The high MTX group also showed some inhibition of cartilage destruction and fibrous tissue. Proliferative effects, and as with the low MTX group, present an active subchondral bone tissue reactive hyperplasia. Conclusion: Tongbiling can effectively inhibit the infiltration of inflammatory cells and fibrous tissue proliferation in cartilage and bone destruction of rheumatoid arthritis model, and reduce and reduce the degree of cartilage destruction so that cartilage structure can maintain close to normal tissue. The form is better than that of MTX and Tripterygium wilfordii and has good treatment and improvement