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以3份加拿大早熟玉米种质为基础材料,利用外引俄罗斯诱导系EMK-1为父本进行人工诱导,通过子粒外观形态鉴定,确定准单倍体子粒。以加拿大早熟群体4诱导的单倍体植株为材料,设置秋水仙素浸种加倍、在6叶期秋水仙素注射加倍与自然加倍3个实验,对单倍体植株进行人工加倍。结果表明,3份加拿大早熟玉米种质单倍体诱导率存在很大差异,诱导率最高达14.37%,最低为5.82%。0.1%浓度的秋水仙素在植株6叶期注射植株加倍的处理效果最好,加倍率为26%;0.3%浓度的秋水仙素浸种12 h处理加倍效果次之,加倍率为14.63%;自然加倍加倍率为3.06%。
Based on 3 Canadian maize germplasms as the basic material, induced by the introduction of the Russian induction line EMK-1 as a male parent artificial induction of particle appearance identification to determine quasi-haploid daughter. The haploid plants induced by Canadian early-maturing population 4 were used as the materials, doubling the soaking of colchicine, double doubling and doubling of the doubling of colchicine at the 6-leaf stage, and double doubling the haploid plants. The results showed that the haploid induction rate of 3 Canadian maize germplasms was very different, the highest induction rate was 14.37% and the lowest was 5.82%. 0.1% concentration of colchicine had the best effect of doubling in 6-leaf stage plants, with a doubling rate of 26%. After the soaking with 0.3% colchicine for 12 hours, the doubling effect was the second, and the doubling rate was 14.63% Double doubling rate of 3.06%.