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目的研究以灵芝、红景天、蝙蝠蛾拟青霉菌粉为主要原料制成的复方中草药胶囊的抗氧化及抗肿瘤作用。方法以不同浓度的复方中草药胶囊进行总抗氧化能力试验(ABTS)、羟自由基清除试验、Hacat细胞内活性氧(ROS)水平试验、Jurkat细胞增殖抑制试验以及Jurkat细胞凋亡试验。结果该复方中草药胶囊具有显著的抗氧化能力,在2μg/ml剂量下,羟自由基清除率可达35.64%±0.32%,并能够显著降低UVB引起的Hacat细胞内的ROS水平(P<0.01);在0.1μg/ml剂量下,可以有效地抑制Jurkat细胞增殖,抑制率可达30%以上,并具有显著的促凋亡作用(P<0.01)。结论该复方中草药胶囊具有一定的抗氧化及抗肿瘤的作用。
Objective To study the anti-oxidation and anti-tumor effects of Chinese herbal medicine capsule with Ganoderma lucidum, Rhodiola sachalinensis and bat moth as raw material. Methods The total antioxidant capacity (ABTS), hydroxyl free radical scavenging assay, Hacat intracellular reactive oxygen species (ROS) level test, Jurkat cell proliferation inhibition test and Jurkat cell apoptosis test were performed in different concentrations of Chinese herbal capsules. Results The compound Chinese herbal capsule had significant anti-oxidative ability. At 2μg / ml, the hydroxyl radical scavenging rate reached 35.64% ± 0.32%, and could significantly reduce the level of ROS in Hacat cells induced by UVB (P <0.01) At 0.1μg / ml dose, Jurkat cell proliferation could be inhibited effectively with the inhibition rate of more than 30% and a significant pro-apoptotic effect (P <0.01). Conclusion The compound Chinese herbal capsule has a certain anti-oxidation and anti-tumor effect.