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目的：建立简便、无毒、灵敏、准确地测定粮食中微量铜的方法。方法：２－ （５－ 溴－ ２－吡啶偶氮）－ ５－ 二乙氨基苯酚（５－ ＢｒＰＡＤＡＰ）光度法。结果：铜含量在０．００～１０．００μｇ 范围内符合比尔定律，Ｙ＝ ２．３４９×１０－ ３＋ ０．０５１４８Ｘ，ｒ＝ ０．９９９９；用本法测定了６ 个样品，回收率为９０．２％ ～１０６．０％ ，平均回收率为９７．８％ ；日间重复试验（７ 次），标准差为０．０１０７～０．０２１５ｍ ｇ／ｋｇ，相对标准偏差为０．５９％ ～１．２９％ 。结论：本法无毒、简便、灵敏，适用于粮食中微量铜的测定。
Objective: To establish a simple, non-toxic, sensitive and accurate method for the determination of trace copper in food. Methods: 2- (5-Bromo-2-pyridylazo) -5-diethylaminophenol (5-BrPADAP) spectrophotometry. Results: The Beer’s law was obeyed in the range of 0.00-10.00μg copper, Y = 2.349 × 10-3 + 0.05148X, r = 0.9999. Six samples were determined by this method and the recoveries were 90.2% ~ 106.0%, the average recovery was 97.8%; the daytime repeated test (7 times), the standard deviation was 0.0107 ~ 0.0215m g / kg, the relative standard deviation was 0.59% ~ 1.29%. Conclusion: This method is non-toxic, simple and sensitive, suitable for the determination of trace copper in food.