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探讨了体外连续培养的人胚肺二倍体细胞在低剂量苯并芘长时期作用下超微结构的早期癌特征。结果发现:低剂量(10~(-7)及10~(-8)mol)苯并芘(Bap)长时期(23～38代)作用下,光镜检查未见细胞有明显癌变征,而电镜下发现细胞核外形不规则,核膜深陷,有者呈复杂折叠及套叠,出现细桥伴分叶核形成,以及核内胞浆包涵体(或称核内假包涵体),核内的常染色质一般丰富;核仁巨大或多核仁等癌细胞特征。说明用电镜可观察到在致癌物作用下细胞早期癌变的征象。实验结果对进一步研究致癌物在低剂量、长时期作用下的致癌机理和预防措施将有重要意义。
The early cancer characteristics of ultrastructure of human embryonic lung diploid cells cultured in vitro under low-dose benzopyrene were explored. The results showed that under light (10~(-7) and 10~(-8) mol) benzopyrene (Bap) for a long period of time (23 to 38 generations), there was no obvious cancerous sign in the light microscope. Electron microscopy revealed that the cell nucleus was irregular in shape, the nuclear membrane was deep-seated, and there were complex folds and nests. The appearance of fine-bridged, leaf-dividing nucleus, and intranuclear cytoplasmic inclusions (or intranuclear pseudo-inclusions) were observed. The euchromatin is generally abundant; the nucleolus is characterized by large or polynuclear cancer cells. Explained by electron microscopy can be observed under the action of carcinogens in early cancer cell signs. The experimental results will be of great significance for the further study of the carcinogenicity of carcinogens under low-dose, long-term carcinogenic mechanisms and preventive measures.