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目的:探索组胺H3受体激动剂(H3R激动剂)IMETIT对豚鼠变应性鼻炎(AR)模型的治疗作用和对P物质(SP)及其受体(SP-R)mRNA表达的影响。方法:以OVA为致敏原,用豚鼠AR模型,观察症状及鼻黏膜病理状况以研究IMETIT对豚鼠AR的治疗作用;免疫组织化学检测鼻中隔黏膜中SP含量,RT-PCR测定鼻中隔黏膜中SP-RmRNA表达以研究IMETIT对SP/SP-R分泌及表达的影响。结果:组胺H3R激动剂IMETIT能有效改善豚鼠AR喷嚏(P<0.01)、鼻痒(P<0.01)、鼻塞(P<0.05),减轻鼻黏膜病理性改变,调低SP/SP-RmRNA分泌及表达(P<0.05或P<0.01)。结论:组胺H3R激动剂IMETIT能有效缓解豚鼠AR的症状,其作用机制与调低SP/SP-R分泌及表达有关。
Objective: To explore the therapeutic effect of IMETIT, a histamine H3 receptor agonist (H3R agonist), on the allergic rhinitis (AR) model in guinea pigs and its effect on the expression of substance P (SP) and its receptor (SP-R) Methods: The effects of IMETIT on guinea pig AR were studied by using guinea pig AR model with OVA as the allergen and the symptoms and pathological conditions of nasal mucosa. The content of SP in mucosa of nasal septum was detected by immunohistochemical method. SP- RmRNA expression to investigate the effect of IMETIT on SP / SP-R secretion and expression. Results: IMETIT, a histamine H3R agonist, can effectively improve AR sneeze (P <0.01), nasal itching (P <0.01), nasal obstruction (P <0.05), reduce the pathological changes of nasal mucosa and decrease the secretion of SP / SP-RmRNA And expressed (P <0.05 or P <0.01). Conclusions: The histamine H3R agonist IMETIT can effectively relieve the symptoms of AR in guinea pigs, and its mechanism may be related to the decrease of the secretion and expression of SP / SP-R.