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目的研究黄芩茎叶总黄酮(TF)对人类单核肿瘤细胞U937脂质代谢的影响。方法取2块24孔板,每孔中接种U937细胞2.5×106个/mL,培养液中含胎牛血清(FCS)10%、LDL 100 mg/L、佛波酯(PMA)100μg/L和不同浓度的TF,根据TF浓度不同分为TF 0,25,50,100,150,200 mg/L 6组,每组8孔,37℃、5%CO2、饱和湿度培养72 h,收集培养细胞测定细胞内总胆固醇(TCH)含量和蛋白质量,细胞涂片油红O染色观察细胞内脂质蓄积情况,测定细胞培养液中Ox-LDL的浓度。结果油红O染色观察,细胞内脂质颗粒随TF浓度的增加而呈减少趋势。TF 0～200 mg/L组细胞内每克蛋白的TCH含量(mg/g)分别为520.13±37.52,375.39±39.78,366.49±35.76,254.26±25.55,263.55±27.72,249.23±22.69,差别具有显著性(F=86.36,P<0.001)。细胞培养液中Ox-LDL浓度分别为0.2113±0.023,0.1938±0.045,0.1288±0.041,0.1238±0.013,0.0850±0.021,0.1429±0.053 mg/mL,差别有极显著性(F=40.73,P<0.0001)。结论 TF可抑制LDL氧化,减少U937细胞内胆固醇的蓄积,抑制泡沫细胞的形成。
Objective To study the effect of total flavonoid (Scutellaria baicalensis stem-leaf total flavonoid) on the lipid metabolism of human monocytic tumor cell line U937. Methods Two 24-well plates were inoculated with 2.5 × 106 cells / mL of U937 cells per well. The medium contained 10% fetal bovine serum (FCS), 100 mg / L LDL, 100 μg / L PMA and Different concentrations of TF were divided into TF 0, 25, 50, 100, 150 and 200 mg / L 6 groups according to the concentration of TF, and each group was cultured in 8 wells, 37 ℃, 5% CO2, saturated humidity for 72 h. The cultured cells were collected for determination of intracellular total cholesterol TCH) content and protein content, cell smear oil red O staining intracellular lipid accumulation, determination of cell culture medium Ox-LDL concentration. Results Oil red O staining showed that intracellular lipid granules showed a decreasing tendency with the increase of TF concentration. TCH content (mg / g) per gram of protein in TF 0 ~ 200 mg / L group was 520.13 ± 37.52,375.39 ± 39.78,366.49 ± 35.76,254.26 ± 25.55,263.55 ± 27.72,249.23 ± 22.69 respectively, the difference was significant Sex (F = 86.36, P <0.001). The concentration of Ox-LDL in cell culture medium was 0.2113 ± 0.023, 0.1938 ± 0.045, 0.1288 ± 0.041, 0.1238 ± 0.013, 0.0850 ± 0.021 and 0.1429 ± 0.053 mg / mL respectively, with the difference being significant (F = 40.73, P <0.0001 ). Conclusion TF can inhibit the oxidation of LDL, reduce the accumulation of cholesterol in U937 cells and inhibit the formation of foam cells.