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目的 研究脑缺血再灌流后热休克蛋白 70 (HSP70 )表达的意义及外源性碱性成纤维细胞生长因子 (b FGF)对其影响。方法 应用免疫组化的方法观察大鼠局灶性脑缺血再灌流后脑组织 HSP70的表达以及在侧脑室注射外源性 b FGF对其影响。结果 缺血 2小时再灌流 0小时即可见 HSP70表达 ,至 2 4小时达高峰。 b FGF组与生理盐水组相比于 6～ 48小时各组可见 HSP70表达增高 (P<0 .0 5 )。结论 脑缺血诱导HSP70的表达 ,外源性 b FGF能增加 HSP70的表达。
Objective To investigate the significance of heat shock protein 70 (HSP70) expression and the effect of exogenous basic fibroblast growth factor (b FGF) on it after cerebral ischemia and reperfusion. Methods Immunohistochemistry was used to observe the expression of HSP70 and the effect of exogenous b FGF injected into the lateral ventricle in rats after focal cerebral ischemia and reperfusion. Results The expression of HSP70 was observed at 0 hour after ischemia for 2 hours and peaked at 24 hours. b Compared with the saline group, the expression of HSP70 in the FGF group was increased (P <0. 05) in all groups from 6 to 48 hours. Conclusions Cerebral ischemia induces the expression of HSP70 and exogenous bFGF can increase the expression of HSP70.