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作者利用氰化钾与血清中的锌和其他干扰离子结合成氰化物,再用水合氯醛使锌离子首先游离出来,与硝基—PAPS 反应呈色。仪器微板读数仪(双波长分光光度计,干涉滤光片577nm 和650nm,半峰宽为7和12nm)MPRA4i 型。微量混合器。微孔板(扁平底、8或16孔)。试剂(1)Tris 缓冲液(0.4mol/L,pH8.1)。(2)KEN 溶液(0.5mol/L)。(3)GT 溶液(pH8.1):盐酸胍基67g,加Tris 缓冲液90ml 液解后,用3mol/L NaOH 或3mol/L HCl 调节至pH8.1,然后用Tris 缓冲液补足至100ml。贮于塑料瓶中,室温至少可稳定一个月。(4)GTAC 溶液:L-抗坏血酸钠盐20mg 溶解于10ml GT 溶液中,加KCN 溶液0.1
The authors make use of potassium cyanide and zinc in serum and other interfering ions to combine into cyanide, then use chloral hydrate to make zinc ion free first and react with nitro-PAPS. Instrument microplate reader (dual wavelength spectrophotometer, interference filters 577nm and 650nm, half-width at 7 and 12nm) MPRA4i type. Micromixer. Microplates (flat bottom, 8 or 16 wells). Reagents (1) Tris buffer (0.4 mol / L, pH 8.1). (2) KEN solution (0.5 mol / L). (3) GT solution (pH8.1): 67 g guanidinium hydrochloride, 90 ml of Tris buffer solution, adjust to pH 8.1 with 3 mol / L NaOH or 3 mol / L HCl, and then make up to 100 ml with Tris buffer. Stored in plastic bottles, at room temperature can be stable for at least a month. (4) GTAC solution: L-ascorbic acid sodium salt 20mg dissolved in 10ml GT solution, add KCN solution 0.1