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为了解血红蛋白与其配合物的结合与解离过程中,各反应分子的结构变化和能量变化的动力学过程以及作用机理,本文利用光声量热法测量了碳氧血红蛋白的光解反应的焓变和结构体积变化。脉冲激光引起碳氧血红蛋白的光解反应,由此激发的声信号即反映光解反应的过程。因此,利用超声换能器测得光解反应产生的声信号,可测定反应过程中的瞬态焓变和结构体积变化等动力学参量。实验中的激发光源为宽度8ns的脉冲激光,光声信号由共振频率为1.5MHz的PZT压电换能器接收。对人、猪、牛、马和兔等哺乳动物的碳氧血红蛋白的光解反应进行了研究,考虑实验系统的时间窗口和碳氧血红蛋白的光解反应中各个过程的弛豫时间,确定实验中检测到的结果对应于寿命为800ns的三级弛豫过程。结果表明,不同物种的血红蛋白在三级弛豫过程中的动力学参量(焓变和结构体积变化)存在明显差异,最后对这种差异的原因进行了分析和讨论。
In order to understand the process of the structural change and energy change of hemoglobin and its complexes, the kinetic process and mechanism of the reaction, the enthalpy change of photolytic reaction of carboxyhemoglobin was measured by photoacoustic calorimetry Structural volume changes. Pulsed laser induced photolysis reaction of carboxyhemoglobin, which stimulated the acoustic signal that reflects the photolytic reaction process. Therefore, using the ultrasonic transducer to measure the acoustic signal generated by the photolysis reaction, the kinetic parameters such as transient enthalpy change and structural volume change during the reaction can be determined. The experimental excitation light source is a pulsed laser with a width of 8ns. The photoacoustic signal is received by a PZT piezoelectric transducer with a resonance frequency of 1.5MHz. The photolysis of carboxyhemoglobin in mammals such as humans, pigs, cattle, horses and rabbits was studied. Taking into account the relaxation time of each process in the time window of the experimental system and the photolytic reaction of carboxyhemoglobin, The detected result corresponds to a three-stage relaxation process with a lifetime of 800 ns. The results showed that there were significant differences in the kinetic parameters (enthalpy change and structural volume change) of hemoglobin in three species during the three stages of relaxation. Finally, the causes of this difference were analyzed and discussed.