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目的:探讨雌二醇联合睾丸酮对乳腺癌MCF-7细胞增殖和凋亡的影响及其可能机制。方法:将10-10mol/L雌二醇和10-5、10-7、10-9和10-11mol/L睾丸酮单独或联合作用于乳腺癌MCF-7细胞24、48和72h,MTT法检测细胞的生长情况,FCM检测细胞周期和细胞凋亡的情况,以及cyclinD1和雄激素受体(androgen receptor,AR)蛋白的表达情况。结果:雌二醇可促进MCF-7细胞的增殖。高浓度(10-5mol/L)睾丸酮可抑制细胞增殖,并抑制雌二醇促细胞增殖的作用;低浓度(10-9mol/L)睾丸酮可促进MCF-7细胞增殖,并增强雌二醇的促细胞增殖作用。雌二醇联合10-5mol/L睾丸酮作用48h后促使细胞周期由G1期进入S期,细胞凋亡率上升,cyclinD1蛋白的表达上调,AR蛋白的表达未见明显变化。结论:雌二醇联合高浓度睾丸酮可抑制乳腺癌细胞的增殖和促进细胞凋亡,可能与上调细胞cyclinD1蛋白的表达有关。
Objective: To investigate the effect of estradiol combined with testosterone on proliferation and apoptosis of breast cancer MCF-7 cells and its possible mechanism. Methods: 10-10mol / L estradiol and 10-5, 10-7, 10-9 and 10-11mol / L testosterone were used alone or in combination on breast cancer MCF-7 cells for 24, 48 and 72 hours. MTT assay FCM was used to detect the cell cycle and apoptosis, as well as the expression of cyclinD1 and androgen receptor (AR) protein. Results: Estradiol promoted the proliferation of MCF-7 cells. Testosterone at high concentrations (10-5mol / L) inhibited cell proliferation and inhibited estradiol-induced cell proliferation. Testosterone at a low concentration (10-9mol / L) promoted MCF-7 cell proliferation and enhanced estradiol Promote cell proliferation. After estradiol combined with 10-5mol / L testosterone for 48h, the cell cycle progressed from G1 phase to S phase. The apoptosis rate increased and the expression of cyclinD1 protein increased. There was no obvious change of AR protein expression. Conclusion: Estradiol combined with high concentration of testosterone can inhibit the proliferation and promote the apoptosis of breast cancer cells, which may be related to the up-regulation of cyclinD1 protein expression.