为验证常染色体畸变是否会引起头颈部鳞癌患者出现第二个原发性肿瘤 ,该文作者采用比较基因组杂交法 (CGH)对 19例头颈部鳞癌患者肿瘤原发部位组织及周围 1cm和 5cm处粘膜组织进行基因检测分析。三处标本组织取出后立即液氮冷冻 ,取标本部分组织切片行苏木素 -伊红染色光镜下检 To determine if autosomal aberrations may cause the appearance of a second primary tumor in a head and neck squamous cell carcinoma, the authors used a comparative genomic hybridization (CGH) method for the primary tumor site and surrounding tissues of 19 patients with head and neck squamous cell carcinoma. Gene detection analysis was performed on mucosal tissues at 1 cm and 5 cm. Immediately after removal of the three specimens, the tissues were frozen in liquid nitrogen, and the tissue sections of the specimens were taken under light microscopy under hematoxylin-eosin staining.