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目的研究细胞因子诱导的杀伤细胞(Cytokine inducedkiller,CIK)对裸鼠腹膜移植人胃癌细胞增殖和凋亡的影响。方法体外制备CIK细胞。建立人胃癌细胞裸鼠腹膜移植小鼠模型,模型鼠腹腔注射CIK细胞,透射电镜观察腹膜移植癌组织形态学变化,流式细胞术(FCM)检测癌细胞增殖周期中G0/G1、S、G2/M期细胞比率、增殖指数(proliferationIndex,PI)和细胞凋亡指数(apoptosisIndex,AI)的变化。结果模型鼠腹膜癌组织细胞的超微结构出现不同程度的凋亡形态学改变,FCM检测DNA直方图上呈现有凋亡峰。G0/G1期细胞比率为(70.73±2.02)%～(75.88±2.23)%,AI为(7.50±2.69)%～(13.16±2.58)%,与对照组相比显著增加,P=0.0000,P=0.008,P<0.01;S期细胞比率为(14.96±1.32)%～(15.34±1.30)%,无显著变化,P>0.05;G2/M期细胞比率为(14.31±1.51)%～(8.91±0.86)%,PI为(29.27±2.05)%～(24.12±2.10)%,均显著降低,P=0.000、0.008、0.033、0.043,P<0.01。随CIK细胞注射剂量的增加和作用时间延长,G0/G1期细胞比率和AI逐渐增加,而G2/M期细胞比率和PI下降,差异均有统计学意义,P<0.05或P<0.01。细胞AI与G0/G1期细胞比率正相关,r=0.676,与PI负相关,r=-0.659。结论CIK细胞可以影响胃癌腹膜种植转移细胞的细胞周期,并具有诱导细胞凋亡的作用。
Objective To investigate the effects of Cytokine induced killer (CIK) on the proliferation and apoptosis of human gastric cancer cell line peritoneal transplantation in nude mice. Methods CIK cells were prepared in vitro. The mouse model of peritoneal transplantation of human gastric cancer cells was established. CIK cells were injected intraperitoneally into the model mice. The morphological changes of the peritoneal transplanted carcinoma tissues were observed by transmission electron microscopy. The cell cycle of G0 / G1, S, G2 / M phase ratio, proliferation index (PI) and apoptosis index (AI). Results The ultrastructure of peritoneal carcinoma cells showed morphological changes of apoptosis in different degree. The apoptosis peak appeared on the FCM test DNA histogram. The percentage of cells in G0 / G1 phase was (70.73 ± 2.02)% ~ (75.88 ± 2.23)% and AI was (7.50 ± 2.69)% ~ (13.16 ± 2.58)%, significantly higher than that in control group (14.31 ± 1.51)% ~ (8.91%) was significantly higher than that in the control group (P = 0.008, P <0.01). The percentage of cells in S phase was (14.96 ± 1.32)% ~ (15.34 ± 1.30)%, ± 0.86)% and PI (29.27 ± 2.05)% ~ (24.12 ± 2.10)%, respectively, all decreased significantly, P = 0.000,0.008,0.033,0.043, P <0.01. With the increase of dose of CIK cells and the prolongation of action time, the percentage of cells in G0 / G1 phase and AI increased gradually, while the cell ratio in G2 / M phase and PI decreased, with statistical significance (P <0.05 or P <0.01). Cell AI was positively correlated with G0 / G1 phase cell ratio, r = 0.676, negatively correlated with PI, r = -0.659. Conclusion CIK cells can affect the cell cycle of metastatic cells in peritoneum of gastric cancer and induce apoptosis.