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目的分析梅州地区儿童社区获得性肺炎中肺炎嗜衣原体感染的流行情况。方法对2013年2月~2014年1月梅州地区社区获得性肺炎患儿均留取咽拭子和血清标本;采用巢氏PCR法检测患儿咽拭子标本中的Cpn主要外膜蛋白基因片段;采用ELISA检测患儿血清中Cpn-Ig M;以上两项检验中一项为阳性即确认为Cpn感染阳性样本。结果 986例患儿中确认为Cpn感染的有143例,阳性率为14.5%;>6岁组感染率最高为28.12%,并与其它组差异有统计学意义;四季均可发病,冬季感染率最高为20.80%,与其它季组差异有统计学意义(P<0.05);巢氏PCR法检出率高于ELISA检测Cpn-Ig M法,但两者一致率较高,差异实际意义不大。结论梅州地区儿童社区获得性肺炎中肺炎嗜衣原体感染好发于冬春季节,感染率男女患儿之间无显著差异,随着年龄的增加,感染率随之增高。
Objective To analyze the prevalence of Chlamydia pneumoniae pneumonia among children with community-acquired pneumonia in Meizhou area. Methods Throat swabs and serum samples were collected from children with community-acquired pneumonia in Meizhou area from February 2013 to January 2014. Nestin’s PCR method was used to detect Cpn major outer membrane protein gene fragments ; Cpn-Ig M in serum was detected by ELISA; one of the two tests was positive, which was confirmed as Cpn-positive samples. Results Of the 986 cases, 143 cases were confirmed as Cpn infection, the positive rate was 14.5%. The highest infection rate of> 6 years old group was 28.12%, and the difference was statistically significant with other groups; (P <0.05). The detection rate of Nestle PCR was higher than that of ELISA by Cpn-Ig M method, but the agreement rate between them was high, and the difference was of little practical significance . Conclusion The infection of Chlamydophila pneumoniae in children with community-acquired pneumonia in Meizhou occurred in winter and spring. The prevalence of infection was no significant difference between boys and girls. With the increase of age, the infection rate increased.