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目的考察不同溶媒对姜黄素在大鼠体内药动学的影响。方法 SD大鼠随机分成2组,分别按剂量200 mg/kg灌胃给予姜黄素水混悬液和姜黄素PEG-400混合溶剂(PEG 400-吐温80-纯水,体积比5∶1∶4)溶液,采用LC-MS/MS法测定血浆中姜黄素的血药浓度,采用DAS2.0软件对数据进行分析。结果LC-MS/MS测定时,姜黄素血药浓度在1~1 000 ng/m L范围内线性关系良好,提取回收率为92.76%~109.75%;灌胃姜黄素水混悬液和姜黄素PEG-400混合溶剂在大鼠体内的Tmax、Cmax等药动学参数之间的差异无统计学意义;在姜黄素水混悬液组出现重吸收峰,致其AUC显著高于姜黄素PEG-400混合溶剂组(提高93%,P<0.05)。结论 LC-MS/MS测定姜黄素血药浓度方法灵敏、重复性良好;2种溶媒不影响大鼠灌胃姜黄素的T_(max)、C_(max);相比PEG-400混合溶剂组,水混悬液组大鼠体内姜黄素吸收明显增多。
Objective To investigate the effects of different solvents on the pharmacokinetics of curcumin in rats. Methods SD rats were randomly divided into two groups. The rats were orally administered with curcumin aqueous suspension and curcumin PEG-400 mixed solvent (PEG 400-Tween 80-pure water, volume ratio of 5: 1: 4) solution, the plasma concentrations of curcumin were determined by LC-MS / MS, and the data were analyzed by DAS2.0 software. Results When the concentration of curcumin in the range of 1 ~ 1 000 ng / m L was determined by LC-MS / MS, the recoveries of curcumin were 92.76% -109.75%. The effects of curcumin aqueous suspension and curcumin PEG-400 mixed solvent in rats Tmax, Cmax and other pharmacokinetic parameters between the difference was not statistically significant; curcumin aqueous suspension reabsorption peak appeared, resulting in AUC was significantly higher than that of curcumin PEG- 400 mixed solvent group (93% increase, P <0.05). Conclusion The method of LC-MS / MS for the determination of curcumin in plasma is sensitive and reproducible. The two vehicles do not affect the T max and C max of curcumin in rats. Compared with PEG-400 mixed solvent group, Curcumin absorption was significantly increased in aqueous suspension group.