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Objective:To amplify antigen genes from patients withhuman immunodeficiency virus type 1 (HIV-1) in GuangdongProvince for candidate AIDS vaccine design.Methods: Viral nucleic acid was isolated from 10 HIV-1infected individuals’ peripheral blood collected during1995-2000 in Guangdong Province. The viral gag p24 geneand env gp120 gene were amplified by nested-PCR andsequenced. The homologies among HIV-1 isolates werecompared with HIV-BLAST.Results: Among 10 HIV-1 isolates, nine are homologous toviruses of subtype B, and one is homologous to viruses ofsubtype E.Conclusion: Subtype B viruses of HIV-1 are predominantlypresent in Guangdong Province.