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目的:研究氨甲酰化促红细胞生成素(CEPO)对毛果芸香碱诱导小鼠癫模型海马神经元的保护作用。方法:C57/B6小鼠随机分为正常未干预组、对照组和CEPO干预组。正常未干预组小鼠不作任何处理;对照组和CEPO干预组小鼠用毛果芸香碱350mg·kg-1腹腔注射诱导癫发作,并于癫发作30min后用地西泮终止发作。对照组和CEPO干预组于诱发癫前2h、癫发作后2h、癫发作后24h分别腹腔注射生理盐水0.1mL和CEPO50μg·kg-1,观察各时间点CEPO干预组与对照组癫发作潜伏期及发作后死亡率;NeuN免疫组化观察各组海马神经元丢失情况。结果:①毛果芸香碱诱导癫发作的成功率为100%(20/20例)。②CEPO干预组癫发作后死亡率与对照组比较差异无统计学意义(P>0.05);CEPO干预组发作潜伏期明显长于对照组(P<0.05)。③NeuN免疫组化显示CEPO干预组海马各区神经元丢失明显少于对照组(P<0.05)。结论:CEPO可以减轻癫的临床发作、抑制神经元凋亡,从而发挥神经保护作用。
AIM: To investigate the protective effect of carbamated erythropoietin (CEPO) on pilocarpine-induced hippocampal neurons in mouse epilepsy model. Methods: C57 / B6 mice were randomly divided into normal non-intervention group, control group and CEPO intervention group. The mice in the normal control group and the CEPO intervention group were intraperitoneally injected with pilocarpine 350 mg · kg-1 to induce epileptic seizure, and the seizures were terminated with diazepam 30 min after the epileptic seizure. In the control group and CEPO intervention group, intraperitoneal injection of saline 0.1 mL and CEPO 50 μg · kg-1 respectively at 2h after induction of epilepsy, 2h after epileptic onset, and 24 h after epileptic seizure were observed in the control group and the CEPO intervention group. Epileptic seizure latency and seizure After death, NeuN immunohistochemistry was used to observe the loss of hippocampal neurons in each group. Results: ① The success rate of pilocarpine induced epilepsy was 100% (20/20 cases). ②CEPO intervention group after epileptic seizures mortality compared with the control group was no significant difference (P> 0.05); CEPO intervention group latency was significantly longer than the control group (P <0.05). ③NeuN immunohistochemistry showed that the neuronal loss in hippocampus in CEPO intervention group was significantly less than that in control group (P <0.05). Conclusion: CEPO can relieve the clinical seizure of epilepsy and inhibit neuronal apoptosis, and thus play a neuroprotective role.