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由豆薯层锈菌(Phakopsora pachyrihzi Syd)引起的大豆锈病是严重影响大豆生长的重要病害之一。防治该病最经济有效的方法之一是抗病育种,而抗病资源的筛选是抗病育种的工作基础。利用室内离体叶片接种方法确定了大豆抗锈接种鉴定的接种浓度,最适接种浓度为100孢子/μL,每片大豆叶子的接种量为2μL,发病时间为第5天,第7天孢子堆破裂释放夏孢子。对296个大豆品种进行了抗锈鉴定,并辅以显微观察和分子检测技术。初筛结果显示:只有大青仁表现出抗性,重复接种鉴定后该品种未表现出明显发病症状,PCR可检测到该品种被接种叶片上的锈菌,针对这个品种再次接种锈菌,结果表现出抗性,其余品种均表现为感病。
Soybean rust caused by Phakopsora pachyrihzi Syd is one of the important diseases that seriously affect the growth of soybean. Prevention and treatment of the disease is one of the most cost-effective disease-resistant breeding, and screening of disease-resistant resources is the basis for disease-resistant breeding. Inoculation concentration was determined by in vitro leaf inoculation method. The optimum inoculation concentration was 100 spores / μL. The inoculation amount of each soybean leaf was 2μL, the onset time was on the 5th day, and on the 7th day, Rupture releases uredospores. 296 soybean varieties were rust-proof, supplemented by microscopic observation and molecular detection techniques. The results of the preliminary screening showed that only Daqingren showed resistance, and no obvious symptom was found after repeated inoculation. The results showed that the rust was inoculated on the leaves by PCR, and the rust was inoculated again to this variety Show resistance, the remaining varieties are manifested as susceptible.