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目的研究丹东地区水体及海(水)产品霍乱弧菌污染情况及疫源性,判断疫源地性质。方法采用霍乱弧菌常规检测方法、多基因PCR方法及脉冲场凝胶电泳方法,分别对鸭绿江水体、港湾海水、沟塘水及各种海(水)产品进行霍乱弧菌检测。结果水样总采样量2672份,检出霍乱弧菌106株,阳性检出率4%。海产品总采样量1353份,检出霍乱弧菌10株,均为小川型非流行株,阳性检出率:0.7%。分离的流行株均为产毒株,O139群为非产毒株。PFGE分析显示所有流行株酶切图谱相似性高,而非流行株图谱的多态性高。结论丹东地区水体已成为霍乱弧菌的自然繁殖地,某些型别,尤其是与从病人分离到的产毒流行株有高度克隆源相关性的霍乱弧菌广泛分布于外环境。
Objective To study the contamination and foci of Vibrio cholerae in water and sea (water) products in Dandong and to determine the nature of foci. Methods Vibrio cholerae was detected in Vibrio cholerae, Vladivostok water, harbor seawater, ditch pond water and various kinds of sea water products by routine Vibrio cholerae detection, polygene PCR and pulsed-field gel electrophoresis. Results A total of 2672 samples were sampled, 106 strains of V. cholera were detected, the positive detection rate was 4%. The total sample of seafood 1353, Vibrio cholerae 10 were detected, are Ogawa non-epidemic strains, the positive detection rate: 0.7%. The isolated strains were all toxigenic and the O139 were non-toxigenic. PFGE analysis showed that the similarity of all the epidemic strains was high, while that of the non-epidemic strains was high. Conclusion Water bodies in Dandong have become a natural breeding ground for Vibrio cholerae. Vibrio cholerae are highly distributed in some environments, especially those with highly clonal origin associated with the toxigenic strains isolated from patients.