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为实现小动物吲哚菁绿(ICG)药代动力学成像,设计了一套高灵敏度扩散荧光层析成像系统。该系统采用了基于离散光纤耦合光子计数采集的仿计算机断层成像扫描方式,在保障高灵敏度和大动态范围测量的前提下,可有效提高系统的空间采样分辨率;同时采用了基于光开关切换四通道串-并混和的测量模式,兼顾了测量时间分辨率和系统性价比之间的平衡。为验证实验系统的有效性,设计了可模拟小动物体内ICG代谢规律的动态仿体,并结合实验室开发的荧光剂药代动力学直接重建算法,实现了ICG代谢速率的重建。实验结果表明,该系统具有较高的空间分辨率、灵敏度和量化性。
In order to achieve small animal indocyanine green (ICG) pharmacokinetic imaging, a high sensitivity diffusion fluorescence tomography system was designed. The system uses a simulated CT scan mode based on discrete fiber coupled photon counting, which can effectively improve the spatial sampling resolution of the system under the premise of ensuring high sensitivity and large dynamic range measurement. At the same time, Channel string - and mixed measurement mode, taking into account the balance between measurement time resolution and system cost. In order to verify the validity of the experimental system, a dynamic flock was designed to simulate the metabolism of ICG in small animals, and the reconstruction of ICG metabolic rate was realized by combining the fluorescence pharmacokinetic direct reconstruction algorithm developed by laboratory. Experimental results show that the system has high spatial resolution, sensitivity and quantification.