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目的:通过观察一次性力竭运动过程中大鼠苍白球内侧部(GPi)神经递质谷氨酸(Glu)和γ-氨基丁酸(GABA)及相关受体表达水平的动态变化,探讨力竭运动过程中苍白球内侧部神经元电活动变化的可能机制。方法:选取雄性Wistar大鼠为实验对象,采用微透析-高效液相色谱联用技术,观察一次性力竭运动过程中大鼠苍白球内侧部神经元胞外Glu和GABA水平动态变化;采用免疫荧光双标技术,检测安静、力竭时和恢复90 min时谷氨酸离子型2B受体(NR2B)和γ-氨基丁酸A型受体α1亚基(GABAAα-1)表达水平。结果:自主运动期大鼠苍白球内侧部神经元胞外Glu/GABA比值均显著下降(P<0.05),而力竭即刻其胞外Glu/GABA比值则显著升高(P<0.01)。与安静状态相比,苍白球内侧部在恢复90 min时NR2B受体阳性细胞数量显著增加(P<0.05),力竭即刻和恢复90 min时GABAAα-1受体阳性细胞数量显著减少(P<0.05,P<0.01)。结论:力竭运动过程中大鼠苍白球内侧部Glu、GABA浓度及NR2B、GABAAα-1受体蛋白表达的改变是导致该核团神经元兴奋性变化的原因之一。
OBJECTIVE: To investigate the dynamic changes of glutamate (Glu) and γ-aminobutyric acid (GABA) and related receptors in the inner part of the globus pallidus (GPi) during one-time exhaustive exercise Possible Mechanisms of Changes in Electrical Activity of Neurons in Inner Pallidus during Exhaustive Exercise. Methods: Male Wistar rats were selected as experimental subjects. The dynamic changes of extracellular Glu and GABA levels in the medial pallidum of rats during one-time exhaustive exercise were observed by microdialysis-high performance liquid chromatography (HPLC) The expression of glutamate ion 2B receptor (NR2B) and γ-aminobutyric acid type A receptor α1 subunit (GABAAα-1) were detected by fluorescence double labeling technique at 90 min after exhaustive exhaustion and recovery. Results: The ratio of extracellular Glu / GABA in medial pallidus was significantly decreased (P <0.05) and the ratio of extracellular Glu / GABA was significantly increased in exhaustive exercise (P <0.01). Compared with resting state, the number of NR2B receptor positive cells in medial part of globus pallidus increased significantly at 90 min (P <0.05), and the number of GABAAα-1 receptor positive cells decreased significantly at 90 min after exhaustive exhaustion (P < 0.05, P <0.01). CONCLUSION: The changes of Glu and GABA concentration and the expressions of NR2B and GABAAα-1 receptor proteins in the medial part of globus pallidus during exhaustive exercise are one of the reasons for the excitability changes of neurons in this nucleus.