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目的寻找一种快速准确、简便易行的鉴别湖北钉螺死活的方法。方法分别用0.05%中性红水溶性染液、0.5%甲基蓝染液、红墨水、伊红亚甲基蓝(MEB)染液、0.4%台盼蓝对活、死钉螺进行染色,染色30 min后压碎螺壳,体视显微镜下观察软体组织着色情况。结果 0.05%中性红可将活钉螺均染成红色,死钉螺均未着色;0.5%甲基蓝染色后,活、死钉螺均不着色;红墨水可将活、死钉螺均染成红色;MEB染液可使部分活、死钉螺着色;0.4%台盼蓝可将活钉螺和部分死钉螺均染成蓝色。结论使用0.05%中性红水溶性染色液对钉螺进行染色处理,可快速、准确、客观地鉴别钉螺死活。
Objective To find a rapid, accurate and easy method to identify the survival of the snail in Hubei province. Methods The live and dead snails were stained with 0.05% neutral red water soluble dye solution, 0.5% methyl blue dye solution, red ink, eosin methylene blue (MEB) dye solution and 0.4% Crushed shell, under the microscope to observe the soft tissue staining. Results 0.05% of neutral red could stain live snails to red, dead snails did not stain; 0.5% methyl blue staining, live and dead snails are not colored; red ink can live and die snails are dyed red; MEB dye can make some live, dead snail coloring; 0.4% trypan blue can live snails and some dead snails are dyed blue. Conclusion Using 0.05% neutral red water-soluble stain to stain Oncomelania snails, it can quickly, accurately and objectively identify the snail.