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抗原决定簇是抗原分子上能识别特定抗体分子上的对位并与之结合的局部结构.乙酰胆碱酯酶(AChE,EC 3.1.1.7)是神经系统的重要水解酶类,主要功能是在中枢及外周神经系统参与神经末梢与下一级神经元或效应器之间的化学传递,催化水解神经递质乙酰胆碱,从而维持神经冲动的正常传导.本研究是采用组合化学多中心多肽合成技术来研究人脑AChE的连续性抗原决定簇以及不同胆碱酯酶的抗原决定簇的差异.人脑尾状核和小脑AChE经亲和层析纯化后,SDS-PAGE还原状态呈一条主带,分子量为67kD.以纯化的人脑AChE为免疫原,免疫Bald/c小鼠,得到小鼠抗人脑AChE抗血清,测定抗体的滴度为12000,以蛋白A亲和层析法纯化得到的多克隆抗体.应用组合化学多中心
Epitopes are local structures on the antigen molecule that recognize and bind to the para-position on a particular antibody molecule Acetylcholinesterase (AChE, EC 3.1.1.7) is an important hydrolytic enzyme of the nervous system and functions primarily in the central The peripheral nervous system participates in the chemical transfer between the nerve endings and the next level of neurons or effectors and catalyzes the hydrolysis of the neurotransmitter acetylcholine so as to maintain the normal conduction of nerve impulses.The present study adopts a combination of chemical multicenter peptide synthesis techniques to study human Brain AChE continuous epitopes and different cholinesterase antigenic determinants differences.After purified by affinity chromatography of human brain caudate nucleus and cerebellum AChE SDS-PAGE showed a primary band with a molecular weight of 67kD Purified human brain AChE was used as immunogen to immunize Bald / c mice to get mouse anti-human brain AChE antiserum, and the titer of the antibody was 12000. The obtained polyclonal antibody was purified by protein A affinity chromatography Application Combination Chemistry Multi-Center