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目的分析疑似登革热病例实验室Ig M抗体检测结果,为有效控制登革热流行提供科学依据。方法收集临床医生报告的疑似登革热病例血清样本,随机采用金标免疫层析法(ICT)或捕捉酶联免疫吸附试验(Mac-ELISA)进行病毒特异性Ig M抗体检测,统计学比较两种检测方法的差异,分析区域内登革热流行特征。结果共检测样本723例,其中ICT法检测样本345例,检出阳性100例,阳性检出率为28.99%;Mac-ELISA法累计检测样本486例,阳性检出120例,阳性检出率24.69%,两种方法的阳性检出率差异无统计学意义(χ2=1.911,P>0.05)。全区18条街道均有阳性病例发生,发病高峰期为10月,20~49岁年龄段为主要发病人群,男女性别比为1:1.12。结论区域内登革热发病集中在人口密集及卫生环境相对较差的区域,阳性病例集中出现在8~11月,中青年为主要发病人群,应适时防控,严防暴发流行;实验室检测结果与采样时间密切相关,ICT检测方法可以作为登革热流行期间早期筛查,及时采集患者血清进行Ig M抗体检测对疫情防控具有重要意义。
Objective To analyze the detection results of IgM antibodies in suspected dengue cases and provide a scientific basis for controlling the epidemic of dengue fever effectively. Methods Serum samples of suspected dengue cases reported by clinicians were collected and virus-specific Ig M antibodies were detected by gold standard immunochromatography (ICT) or enzyme-linked immunosorbent assay (Mac-ELISA). The results of two tests Method of difference, analyze the epidemic characteristics of dengue in the area. Results A total of 723 samples were detected, of which 345 samples were detected by ICT method and 100 samples were positive, the positive rate was 28.99%. 486 samples were detected by Mac-ELISA, 120 samples were positive, the positive detection rate was 24.69 %, There was no significant difference between the two methods (χ2 = 1.911, P> 0.05). The 18 districts of the district have positive cases, the peak incidence of October, 20 to 49 age group as the main incidence of the crowd, male to female ratio was 1: 1.12. Conclusions The incidence of dengue in the area is concentrated in the areas with dense population and relatively poor sanitation environment. The positive cases are concentrated in 8 ~ 11 months. The middle-aged and young people are the main pathogenetic groups. Prevention and control should be timely to prevent outbreaks. The laboratory test results and sampling Time is closely related to ICT detection methods can be used as early screening during the epidemic of dengue, timely collection of patient serum IgM antibody detection is of great importance to prevention and control of the epidemic.