论文部分内容阅读
建立了液相色谱-串联质谱(LC-MS/MS)法测定SD大鼠心、肝、脾、肺、肾、骨骼肌、小肠、性腺(睾丸、卵巢)、脂肪和脑10种组织中的银杏内酯B。以白果内酯为内标,采用ESI源负离子模式、多反应监测(MRM)进行定量分析,监测离子对m/z 423.1→m/z 367.1(银杏内酯B)和m/z 325.2→m/z 163.2(白果内酯)。以大鼠肝组织作为代表性基质进行方法学验证,结果表明银杏内酯B在20~5 000 ng/g范围内线性关系良好,方法回收率90.26%~102.99%,日内、日间RSD小于8.6%。采用该法考察雄性SD大鼠尾静脉注射银杏内酯B注射液(10 mg/kg)后的组织分布情况,结果显示,各组织中的药物浓度随着给药时间的延长而迅速衰减;同时该药更易蓄积在血流量较大的肝肾组织中。此外,该药可穿透血脑屏障进入脑组织。
A liquid chromatography-tandem mass spectrometry (LC-MS / MS) method was developed for the determination of 10 kinds of tissues in heart, liver, spleen, lung, kidney, skeletal muscle, small intestine, Ginkgolide B Using bilobalide as internal standard, ESI source negative ion mode and multiple reaction monitoring (MRM) were used for quantitative analysis. The effects of ion pair m / z 423.1 → m / z 367.1 (ginkgolide B) and m / z 325.2 → m / z 163.2 (bilobalide). Methodological validation of rat liver tissue as a representative matrix showed that ginkgolide B had a good linearity within the range of 20-5 000 ng / g with a method recovery of 90.26% -102.99% and a daily RSD of less than 8.6 %. The method was used to investigate the tissue distribution of male SD rats injected with ginkgolide B (10 mg / kg) through the tail vein. The results showed that the concentration of drug in each tissue decreased rapidly with the prolongation of administration time. The drug is more likely to accumulate in the liver and kidney tissue with larger blood flow. In addition, the drug penetrates the blood-brain barrier into the brain.