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目的探讨男性不育症患者Y染色体微缺失分子检测临床意义。方法应用多重PCR对178例不育症患者进行Y染色体AZFa、AZFb和AZFc基因微缺失检测。结果41例特发性无精症患者中有10例缺失,占24.3%;34例严重少精子症患者中有4例缺失,占11.7%;其余103例少弱精子症患者中没有检出缺失。结论在男性不育症患者中,Y染色体AZF基因微缺失是特发性无精子或严重少精子症发生的重要原因,基因检测可为正确诊断和合理治疗提供科学依据。
Objective To investigate the clinical significance of detection of Y chromosome microdeletions in male infertility patients. Methods Multiplex PCR was used to detect the gene deletion of AZFa, AZFb and AZFc in 178 infertile patients. RESULTS: Of the 41 cases of idiopathic azoospermia, 10 were missing, accounting for 24.3%; 4 of 34 patients with severe oligozoospermia were deleted, accounting for 11.7%; and the remaining 103 cases of oligozoospermia were not detected . Conclusion In the male infertility patients, Y chromosome AZF gene microdeletions is an important cause of idiopathic azoospermia or severe oligospermia. Gene detection can provide a scientific basis for correct diagnosis and rational treatment.