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为了寻找方便、安全、高效的小麦单倍体苗加倍处理方法,选用秋水仙素3个浓度(0.1%、0.2%、0.3%)、3个处理时间(3h、4h、5h)对单倍体苗进行浸根加倍处理;同时,比较了加光与不加光、全程通气与不通气、分蘖节打孔与不打孔等技术措施下单倍体苗的移栽成活率、加倍结实率。结果表明,室温下以0.2%的秋水仙素浸根处理3~4h,期间持续通入空气并进行遮光处理的效果最佳,可获得90.0%的加倍率。避光和持续通气处理有利于提高成活率和加倍率,其在高浓度加倍处理和长时间加倍处理时显得尤其重要。分蘖节打孔伤害了植株,降低了加倍后植株的成活率,但能提高低浓度短时间处理时植株的加倍率,对低浓度长时间处理和高浓度处理则不适用。
In order to find a convenient, safe and efficient wheat haploid seedling doubling treatment, three concentrations of colchicine (0.1%, 0.2%, 0.3%), three treatments (3h, 4h, 5h) The seedlings were treated with double root immersion treatment. At the same time, the transplanting survival rate and doubling seed setting rate of haploid seedlings were compared under different technical measures such as light and no light, full aeration and no aeration, perfunctory and non-perforated tillers. The results showed that when rooted at 0.2% colchicine for 3 ~ 4 h at room temperature, the effect of continuous aeration and shading treatment was best, and the doubling rate of 90.0% could be obtained. Dark and continuous aeration treatment is conducive to improve the survival rate and magnification, it is especially important in high concentration doubling treatment and long time doubling treatment. The tillering node punctured the plants and reduced the doubling rate of the survival rate of the plants, but increased the doubling rate of the plants when treated with the low concentration for a short time, which was not suitable for the low-concentration long-term treatment and the high-concentration treatment.