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目的利用四氧化三铁(Fe_3O_4)纳米粒作为模型,研究粒径对纳米粒子靶向性的影响,同时考察不同粒径Fe_3O_4的光热治疗效果。方法本研究采用水热法合成50 nm(Fe_3O_4-50)和210 nm(Fe_3O_4-210)两种粒径的Fe_3O_4纳米粒,考察其光热升温效果、细胞摄取情况和各水平磁响应性;评价外磁场介导下纳米粒在荷瘤小鼠中体内分布情况,并开展体内光热治疗。结果所制备的Fe_3O_4纳米粒形貌均一。两种纳米粒在体外具有近似的光热转化效率,近红外光照射5 min后,两组溶液均从25°C升至72°C;Fe_3O_4-50细胞摄取水平较高,对细胞的光热杀伤效果较好,1.5 W·cm~(-2)照射3 min后细胞存活率低于60%,而相同条件下,Fe_3O_4-210的细胞存活率高于75%。体内分布结果显示,Fe_3O_4-210具有更好的肿瘤靶向及治疗效果,照射3 min后,肿瘤部位温度达到47.3℃,而Fe_3O_4-50温度低于40℃。结论两种粒径Fe_3O_4均具有良好的光热效果。小粒径Fe_3O_4(50 nm)具有较高的细胞摄取效果;大粒径Fe_3O_4(210 nm)具有更好的肿瘤靶向能力,更适合于肿瘤热消融治疗。
OBJECTIVE To study the effect of particle size on the targeting of nanoparticles by using Fe3O4 nanoparticles as model, and to examine the photothermal therapeutic effect of Fe3O4 nanoparticles with different particle sizes. Methods The hydrothermal method was used to synthesize Fe_3O_4 nanoparticles of 50 nm (Fe_3O_4-50) and 210 nm (Fe_3O_4-210), and their thermal and photothermal effects, cellular uptake and magnetic response were evaluated. External magnetic field-mediated nanoparticles in tumor-bearing mice in vivo distribution, and to carry out photothermal therapy in vivo. Results The prepared Fe_3O_4 nanoparticles were uniform in morphology. Both nanoparticles had similar photothermal conversion efficiency in vitro. After 5 min of irradiation with near-infrared light, both solutions rose from 25 ° C to 72 ° C. Fe_3O_4-50 cells had higher uptake, The killing effect was better. The cell survival rate was lower than 60% after irradiated with 1.5 W · cm ~ (-2) for 3 min. Under the same conditions, the cell survival rate of Fe 3 O 4-210 was higher than 75%. The results of in vivo distribution showed that Fe 3 O 4-210 had better tumor targeting and therapeutic effects. After 3 min irradiation, the temperature of the tumor reached 47.3 ℃, while the temperature of Fe 3 O 4-50 was lower than 40 ℃. Conclusion Both Fe_3O_4 particles have good photothermal effect. Small particle size Fe 3 O 4 (50 nm) has higher cell uptake efficiency. Large size Fe 3 O 4 (210 nm) has better tumor targeting ability and is more suitable for tumor thermal ablation.