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目的探讨病毒性脑膜脑炎患儿脑脊液(CSF)细胞学检测和细胞因子的意义。方法将79例病毒性脑膜脑炎患儿按病情分为轻症组和重症组,采用腰椎穿刺术采集CSF进行常规和细胞因子检测,另取CSF检测正常的64例儿童为对照组,比较3组CSF细胞学检测和生化检测结果。结果对照组、轻症组和重症组CSF淋巴细胞阳性率分别为12.5%、63.41%和86.11%,TNF-α水平分别为(6.37±1.48)mg.L-1、(22.75±6.68)mg.L-1和(53.41±7.74)mg.L-1,3组间比较差异有统计学意义(P<0.01);轻症组和重症组CSF单核细胞和神经元特异性烯醇化酶阳性率均显著高于对照组,但2组间差别无统计学意义。结论 CSF淋巴细胞数和TNF-α可作为脑损伤轻重程度的判定标准,单核细胞和神经元特异烯醇化酶也可作为脑损伤的依据之一。
Objective To investigate the significance of CSF cytology and cytokines in children with viral meningoencephalitis. Methods 79 children with viral meningoencephalitis were divided into mild group and severe group according to their illness. CSF was collected by lumbar puncture for routine and cytokine detection. Another 64 children with normal CSF test were used as control group. CSF cytology test and biochemical test results. Results The positive rates of CSF were 12.5%, 63.41% and 86.11% in the control group, mild and severe cases respectively, and the levels of TNF-α were (6.37 ± 1.48) mg.L-1 and (22.75 ± 6.68) mg, respectively. L-1 and (53.41 ± 7.74) mg.L-1 and 3, respectively (P <0.01). The positive rates of CSF monocyte and neuron-specific enolase in mild and severe cases Were significantly higher than the control group, but no significant difference between the two groups. Conclusions The number of CSF lymphocytes and TNF-α can be used as the criterion of severity of brain injury. Monocytes and neuron-specific enolase can also be used as a basis for brain injury.